Descriptive Analysis the Analgesic Activity of Chloroform Fraction from Acorus Calamus L. Leaf Extract on Male White Mice (Mus Musculus) Induced by Acetic Acid
DOI:
https://doi.org/10.58471/health.v2i01.110Keywords:
Jeringau Leaf Extract, Analgesic Activity, Chloroform Fraction, Male White Mice, Pain ManagementAbstract
Pain management is a critical aspect of pharmaceutical research, and Acorus calamus L., commonly known as jeringau, has garnered attention for its potential analgesic properties. This study focuses on evaluating the analgesic activity of the chloroform fraction from ethanol extract of jeringau leaves (Acorus calamus L.) on male white mice induced by acetic acid. Chronic and acute pain significantly impacts individuals' quality of life, emphasizing the need for effective and safe analgesic alternatives. While Acorus calamus leaf extract has shown pharmacological potential, especially the chloroform fraction, a dedicated investigation into its analgesic effects on animal models is lacking. Male white mice, recognized for their reproducible and measurable responses to painful stimuli, serve as relevant subjects in this study. The population of 25 mice, aged 2-3 months and weighing 20-30 grams, is divided into 5 treatment groups, including negative and positive controls. The study employs three dosages of the chloroform fraction, administered orally, with observations made on writhing behavior following intraperitoneal injection of 0.5% acetic acid. Statistical analysis will evaluate significant differences between treatment groups, providing insights into the analgesic potential. Results indicate that Dosage III (44.8 mg/40g BW) exhibits the highest analgesic efficacy at 73.99%, showcasing a dose-dependent response. The mechanism involves flavonoids inhibiting the cyclooxygenase enzyme, aligning with established analgesic actions. Ibuprofen, the positive control, and jeringau leaf extract demonstrate analgesic effects, reinforcing traditional knowledge. This research contributes to scientific understanding, establishing a foundation for further exploration.
References
Akbar, S. (2020). Handbook of 200 medicinal plants: a comprehensive review of their traditional medical uses and scientific justifications.
Ardianto, N., Prasetyawan, F., Saristiana, Y., Muslikh, F. A., Mildawati, R., Dhafin, A. A., ... & Rofiq, A. (2023). Forensic Pharmacy Case Study: Identification of Hazardous Mercury Content as a Whitening Agent in Beauty Cream Products. International Journal of Contemporary Sciences (IJCS), 1(2), 85-90.
Elshikh, M. S., Rani, E., Al Farraj, D. A., Al-Hemaid, F. M., Gawwad, M. R. A., Malar, T. J., ... & Vijayaraghavan, P. (2022). Plant secondary metabolites extracted from Acorus calamus rhizome from Western Ghats, India and repellent activity on Sitophilus oryzae. Physiological and Molecular Plant Pathology, 117, 101743.
Fitzcharles, M. A., Cohen, S. P., Clauw, D. J., Littlejohn, G., Usui, C., & Häuser, W. (2021). Nociplastic pain: towards an understanding of prevalent pain conditions. The Lancet, 397(10289), 2098-2110.
Glare, P., Overton, S., & Aubrey, K. (2020). Transition from acute to chronic pain: where cells, systems and society meet. Pain Management, 10(6), 421-436.
Holifah. (2019). Analgesic Activity of Ethanol Extract of Jeringau Leaves (Acorus calamus L.) in Mice Induced by Acetic Acid. Thesis.
Islam, M. M., Farag, E., Mahmoudi, A., Hassan, M. M., Atta, M., Mostafavi, E., ... & Mkhize-Kwitshana, Z. (2021). Morphometric study of Mus musculus, Rattus norvegicus, and Rattus rattus in Qatar. Animals, 11(8), 2162.
Kovalenko, A. M., Tkachev, A. V., Tkacheva, O. L., Gutyj, B. V., Prystupa, O. I., Kukhtyn, M. D., ... & Kotelevych, V. A. (2020). Analgesic effectiveness of new nanosilver drug. Ukrainian Journal of Ecology, 10(1), 300-306.
Muhammad. (2012). Farmakologi dan Terapi. Jakarta: Salemba Medika.
Prasetyawan, F., & Saristana, Y. (2023). Coronavirus Desease 2019 (COVID-19) Dan Penyakit Ginjal Kronis (PGK): Tinjauan Prevalensi, Faktor Risiko dan Pengobatan Pertama. DEWA Publising.
Prasetyawan, F., Saristiana, Y., Febriyana, R., & Ananta, S. C. (2022). Evaluasi Perbandingan Penggunaan Oseltamivir Dengan Favirapir Terhadap Outcome Terapi Pasien Covid-19 di Rumah Sakit Umum Daerah Gambiran Kota Kediri. Java Health Jounal, 9(1).
Prawanayoni, S. S., & Sudirga, S. K. (2020). Isolasi dan identifikasi senyawa antijamur daun jeringau (Acorus calamus Linn.) sebagai pengendali jamur Athelia rolfsii Sacc. penyebab penyakit busuk batang pada tanaman kedelai. Jurnal Metamorfosa, 7(2), 152-158.
Saristiana, Y., Prasetyawan, F., Mildawati, R., Muslikh, F. A., Dhafin, A. A., Raharjo, S. M., ... & Rofiq, A. (2023). Farmakoantartika Literature Study: Bioactivity of Pagodroma nivea on Fossil Deposits from Mujito. International Journal of Contemporary Sciences (IJCS), 1(2), 91-98.
Setiaji, J., Hariwitonang, J., & Johan, T. I. (2022). Antibacterial Activity of Ethanolic Extracts from Jeringau (Acorus calamus). In IOP Conference Series: Earth and Environmental Science (Vol. 1118, No. 1, p. 012006). IOP Publishing.
Sharma, V., Sharma, R., Gautam, D. S., Kuca, K., Nepovimova, E., & Martins, N. (2020). Role of Vacha (Acorus calamus Linn.) in neurological and metabolic disorders: evidence from ethnopharmacology, phytochemistry, pharmacology and clinical study. Journal of clinical medicine, 9(4), 1176.
Susanty, A., dkk. (2014). Pemisahan dan Identifikasi Komponen Minyak Atsiri Daun Sirih Hijau (Piper betle L.) Varietas Biak. Jurnal Kimia Sains dan Aplikasi, 17(1), 1-5.
Wang, J. F., Zhu, C. Y., Weng, B. S., Mo, P. W., Xu, Z. J., Tian, P., ... & Bai, J. H. (2021). Regulation of heavy metals accumulated by Acorus calamus L. in constructed wetland through different nitrogen forms. Chemosphere, 281, 130773.
